A proteomics-based approach towards identifying host and pathogen proteins critical to clubroot establishment in canola

Term:

4 years, beginning in 2020

Status:

Ongoing

Researcher(s):

Chris Todd, University of Saskatchewan

SaskOilseeds Investment:

$250,010

Total Project Cost:

$500,020

Funding Partners:

Western Grains Research Foundation

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Project Description

The proposed research will deliver knowledge and tools to improve utilization of existing clubroot resistant cultivars and to accelerate the discovery of new clubroot resistance genes, with the anticipation of exploring broad-spectrum and durable clubroot resistance that will be highly beneficial to breeders and growers of canola and other Brassica crops.

Objectives

  1. Identification of Pb effectors using a BiolD-based method in Arabidopsis.
  2. Screen of putative Pb effectors in transgenic Arabidopsis.
  3. Targeted analysis of root hair cell (RHC) and root cortical cell (RCC) proteomes of clubroot resistant and susceptible canola.
  4. Assessment of differentially expressed canola proteins for association with resistance/susceptibility to clubroot.
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