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Addressing yield stability drivers of canola in a changing climate using high throughput phenotyping

The tools developed and verified through this project will enable efficient development of resilient varieties. The results support potential of canola digital phenotypes to field-scale agronomic applications. The expansive data sets and samples generated through this project are and will be used in various research projects, extending the utility of grower-invested research dollars.

Pre-breeding lines combining canola quality with sclerotinia resistance, good agronomy and genomic diversity from PAK93

Seed of PAK93-based pre-breeding lines will be more attractive to plant breeding companies than the original germplasm such as PAK54, primarily because it will be faster to develop hybrid varieties that combine the desirable traits from PAK93-derived lines with other important traits such as herbicide tolerance and resistance to the diseases, blackleg and clubroot.

Enabling Canola Protein Optimization (ECPO)

Increase canola protein inclusion rates in monogastric animal feeds, followed by canola germplasm that produces protein better suited for human diets, and finally specialty varieties that produce protein for specific technical applications.

A proteomics-based approach towards identifying host and pathogen proteins critical to clubroot establishment in canola

The proposed research will deliver knowledge and tools to improve utilization of existing clubroot resistant cultivars and to accelerate the discovery of new clubroot resistance genes, with the anticipation of exploring broad-spectrum and durable clubroot resistance that will be highly beneficial to breeders and growers of canola and other Brassica crops.

Cloning clubroot resistance genes from B. nigra and transferring the genes into canola through a CRISPR/Cas9 based technology

The pathogen (P. brassicae) populations in western Canada is evolving rapidly. It is extremely important to have canola cultivars with new sources of resistance avialabe to canola producers in Saskatchewan.

WCVM Research Chair in Pollinator Health

The Western College of Veterinary Medicine (WCVM) was the first veterinary college in North America to begin a honey bee research and teaching program, recognizing the high importance of pollinators for the long-term sustainability of agriculture and food security.

Development of an Insect Quarantine and Rearing Facility

This facility will increase the overall capacity for insect and pest related research and will therefore benefit numerous researchers and projects at the USask. Finally, this facility will result in important training opportunities.

Purifying genotypes of Plasmodiophora brassicae and developing SNP markers linked to races of P. brassicae populations collected in western Canada

Pathotying with the CCD or race profiling with the NILs is a phenotyping based approach, which can be tedious and time consuming. A genotyping based method could be an ultimate solution for race profiling.

Investigating interactions of ascospores and pycnidiospores with blackleg resistance in canola and efficacy of seed applied fungicides in these specific interactions in western Canada

This is the first investigation into the infection by pycnidio- and asco-spores, as well as a mixture via wounds under the influence of cultivar resistance and fungicide seed treatment for blackleg disease in canola. The findings from this study suggested that it is feasible to produce ascospores and pycnidiospores in the lab and use these types of spores to test canola materials for blackleg resistance in both controlled environment and field conditions.

New Clubroot Pathotypes and Second Generation Resistance

Clubroot poses a significant threat to canola growers. Although genetic resistance is critical for managing the disease, changes in pathogen virulence endanger its effectiveness. Second-generation resistance offers promise against pathotypes that can overcome first-generation resistance. However, without an understanding of pathogen virulence on second-generation resistance, we risk compromising its efficacy. This project provided data on the performance of second-generation clubroot-resistant cultivars in the field, along with insights into the virulence characteristics of pathogen populations collected from these cultivars.

Enhanced understanding of cleavers populations in Western Canada

Understanding weed biology is the first BMP for reducing risk of herbicide resistance. This study will result in an understanding of different cleavers biotypes that exist across the Prairies, their emergence phenology, and some of their biological characteristics.

Collecting the carbon data needed for Climate-Smart agriculture in Saskatchewan

This work will provide crucial information needed by the agricultural industry and growers: field-scale data on how farmers may best balance agronomic and environmental outcomes by adjusting either the timing of fertilizer application or the fertilizer formulation.